Journal: Science Advances

Article Title: Cancer-associated SF3B1-K700E mutation controls immune responses by regulating T reg function via aberrant Anapc13 splicing

doi: 10.1126/sciadv.ado4274

Figure Lengend Snippet: ( A ) Representative image (left) and weight (right) of the spleens from indicated mice implanted with AML. ( B ) The percentage of GFP + AML cells in peripheral blood (PB) of indicated mice at different times after implantation of AML ( n = 7 per genotype per group). ( C ) Kaplan-Meier curves showing the survival of the indicated mice implanted with AML ( n = 7 per genotype per group). ( D and E ) Representative flow cytometric analysis (left) and percentage (right) of GFP + AML cells in the PB (E) and bone marrow [BM, (F)] from indicated mice 21 days after AML implantation. Boxed area: cell population of interest. Data are from three experiments [(A) and (B); (C) to (E), right panels, presented as means ± SEM] or are from one representative of three independent experiments [(C) to (E), left panels]. The statistical significance for (B) was determined using the log-rank (Mantel-Cox) test. * P < 0.05 and ** P < 0.01 (two-tailed Student’s t test).

Article Snippet: IRF4-MIEG-GFP was a gift from M. H. Kaplan Laboratory (Indiana University School of Medicine); 7×E-Box:Renilla (plasmid #124532) and MSCV-PIG-Myb (plasmid #66988) were purchased from Addgene. pGL3-promoter (plasmid #E1761) was purchased from Promega (Madison, WI).

Techniques: Two Tailed Test

Journal: iScience

Article Title: Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosis

doi: 10.1016/j.isci.2022.104123

Figure Lengend Snippet:

Article Snippet: Renilla reporter , Addgene , RRID:Addgene_62186.

Techniques: Recombinant, Sequencing, Reporter Assay, Control, Software, Imaging, Microinjection, Inverted Microscopy